{"id":22592,"date":"2025-09-24T12:35:04","date_gmt":"2025-09-24T16:35:04","guid":{"rendered":"https:\/\/www.arsacs.com\/?p=22592"},"modified":"2026-03-16T18:18:10","modified_gmt":"2026-03-16T22:18:10","slug":"structural-determination-of-sacsin-dr-walid-houry-4","status":"publish","type":"post","link":"https:\/\/2026.arsacs.com\/fr\/structural-determination-of-sacsin-dr-walid-houry-4\/","title":{"rendered":"\u201cStructural Determination of Sacsin\u201d &#8211; Dr. Walid Houry"},"content":{"rendered":"<p>Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) was described in 1978 as a unique form of ataxia accompanied with motor speech anomalies, muscle wasting phenotypes, and peripheral nerve complications. Genetic studies have established that ARSACS is caused by mutations in sacsin \u2013 a protein whose biology is poorly known. Current research suggests that sacsin supports cytoskeletal and mitochondrial organization. To shed light on sacsin function and to support the future development of potential therapies, the structure of sacsin in both native and mutated states needs to be determined. However, no published research has described the purification of sacsin, a 520 kDa protein, for <em>in vitro<\/em> and structural investigations. Our group is currently working to optimize the conditions for the expression and purification of sacsin. We are also working to obtain its three-dimensional (3D) structure using cryogenic electron microscopy.<\/p>\n<p>Grant: $100,000<\/p>\n<p>Duration : one year<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignleft  wp-image-6572\" src=\"https:\/\/2026.arsacs.com\/wp-content\/uploads\/2021\/02\/Walid_Houry_University_Toronto-e1606423339816.jpg\" alt=\"\" width=\"200\" height=\"236\" \/><\/p>\n<p>Dr. Walid A. Houry, <br \/>Department of Biochemistry<br \/>Temerty Faculty of Medicine, University of Toronto<br \/>661 University Avenue, MaRS Centre, West Tower, Room 1612<br \/>Toronto, ON Canada M5G 1M1<\/p>\n<p>Contact:\u00a0<a href=\"mailto:walid.houry@utoronto.ca\">walid.houry@utoronto.ca<\/a><\/p>\n\n\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) was described in 1978 as a unique form of ataxia accompanied with motor speech anomalies, muscle wasting phenotypes, and peripheral nerve complications. Genetic studies have established that ARSACS is caused by mutations in sacsin \u2013 a protein whose biology is poorly known. Current research suggests that sacsin supports [&#8230;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[],"class_list":["post-22592","post","type-post","status-publish","format-standard","hentry","category-current"],"_links":{"self":[{"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/22592","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/comments?post=22592"}],"version-history":[{"count":6,"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/22592\/revisions"}],"predecessor-version":[{"id":25125,"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/22592\/revisions\/25125"}],"wp:attachment":[{"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/media?parent=22592"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/categories?post=22592"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/2026.arsacs.com\/fr\/wp-json\/wp\/v2\/tags?post=22592"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}